Recombinant Apis mellifera carnica Defensin-1 | CSB-EP686329ADAM
- 3 - 7 Working Days
Recombinant Apis mellifera carnica Defensin-1 | CSB-EP686329ADAM | Cusabio
Alternative Name(s): Defensin-1
Gene Names: N/A
Research Areas: Others
Organism: Apis mellifera carnica (Carniolan honeybee)
AA Sequence: VTCDLLSFKGQVNDSACAANCLSLGKAGGHCEKGVCICRKTSFKDLWDKRF
Tag Info: N-terminal 6xHis-KSI-tagged
Expression Region: 44-94aa
Sequence Info: Full Length of Mature Protein
MW: 20.9 kDa
Purity: Greater than 85% as determined by SDS-PAGE.
Relevance: Found in royal jelly and in hemolymph, potent antibacterial protein against Gram-positive bacteria at low concentration.
Reference: "Silencing of Apis mellifera dorsal genes reveals their role in expression of the antimicrobial peptide defensin-1." Lourenco A.P., Florecki M.M., Simoes Z.L.P., Evans J.D. Insect Mol Biol 27:577-589(2018)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Involvement in disease:
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: Q5J8R1
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: N/A
STRING Database Link: N/A
OMIM Database Link: N/A