Recombinant Apis mellifera Venom serine carboxypeptidase | CSB-EP509855DNK

Recombinant Apis mellifera Venom serine carboxypeptidase | CSB-EP509855DNK | Cusabio

Alternative Name(s): Allergen: Api m 9

Gene Names: N/A

Research Areas: Others

Organism: Apis mellifera (Honeybee)

AA Sequence: FTNVYPKPKYCPLLHEEDAGIPLFLTPLIENGKIDEARNKAVIQHKEVEAISSYAGFLTVNKKYNSNMFFWFFPALHDPKTAPVVLWLQGGPGATSMYGLFLENGPFIVTKNKTLKMREYSWNKCHNLLYIDNPVGTGFSFTEDERGYATNETHVGRDVHTALVQFFELFPELQTNDFYVTGESYGGKYVPAVSHAIKDYNIKAKIKINLKGLAIGNGLTDPVNQLDYGDYLYQLGLLDANGRNLFQKYEEQGKNLIKQEKWLEAFDLFDELLDGDITQQPSLYKNLTGFDYYFNYLHEKDPSNDSDYMVEWLQRADVRKAIHVGNRTFIPESKKVEKYMKADVMQSLAVLIADLTQHYRVLIYNGQLDIIVAYPLTENYLQKLKWPGAEKYKTAQRKVWFVGNELAGYSKTVDSLTEVLVRNAGHMVPLDQPKWALDLITRFTHNKGF

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 19-467aa

Sequence Info: Full Length of Mature Protein

MW: 67.6 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Release of a C-terminal amino acid with broad specificity.

Reference: "Identification, recombinant expression and characterization of high molecular weight hymenoptera venom allergens."Blank S., Seismann H., Braren I., Bockisch B., Bredehorst R., Ollert M., Grunwald T., Spillner E.Submitted (FEB-2009)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function:

Involvement in disease:

Subcellular Location: Secreted

Protein Families: Peptidase S10 family

Tissue Specificity: Expressed by the venom duct.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: C9WMM5

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A