Cusabio Polyclonal Antibodies
Histone H3R8me2s Antibody | CSB-PA043446
- SKU:
- CSB-PA043446
- Availability:
- 3 to 7 Working Days
- Size:
- 100ul
Description
Histone H3R8me2s Antibody | CSB-PA043446 | Cusabio
Histone H3R8me2s Antibody is Available at Gentaur Genprice with the fastest delivery.
Online Order Payment is possible or send quotation to info@gentaur.com.
Product Type: Polyclonal Antibody
Target Names: HIST3H3
Aliases: HIST1H3J; H3/j; 3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;
Background: Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1) . The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5) . These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6) . In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4, 7) . Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2, 3) . Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10) . Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11) .
Isotype: IgG
Conjugate: Non-conjugated
Clonality: Polyclonal
Uniport ID: Q16695
Host Species: Rabbit
Species Reactivity: Human, Mouse, Rat
Immunogen: A synthetic methylated peptide corresponding to residues surrounding R8of human histone H3
Immunogen Species: Human
Applications: ELISA, WB, IF, IP
Tested Applications: ELISA, WB, IF, IP;WB:1:500-1:2000, IF:1:50-1:200, IP:1:50-1:200
Purification Method: Antigen Affinity Purified
Dilution Ratio1: ELISA:1:2000-1:10000
Dilution Ratio2: WB:1:500-1:2000
Dilution Ratio3: IF:1:50-1:200
Dilution Ratio4: IP:1:50-1:200
Dilution Ratio5:
Dilution Ratio6:
Buffer: Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: liquid
Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Initial Research Areas: Epigenetics and Nuclear Signaling
Research Areas: Epigenetics & Nuclear Signaling
