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Histone H4 Antibody | CSB-RA010429A0HU

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CSB-RA010429A0HU
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  • Histone H4 Antibody
  • IHC image of CSB-RA010429A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-RA010429A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Overlay histogram showing Hela cells stained with CSB-RA010429A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
€373.00 - €555.00
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Description

Histone H4 Antibody | CSB-RA010429A0HU | Cusabio

Histone H4 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Antibody Type: Recombinant Antibody

Target Names: HIST1H4A

Aliases: Histone H4, HIST1H4A, H4/A, H4FA, AND, HIST1H4B, H4/I, H4FI, AND, HIST1H4C, H4/G, H4FG, AND, HIST1H4D, H4/B, H4FB, AND, HIST1H4E, H4/J, H4FJ, AND, HIST1H4F, H4/C, H4FC, AND, HIST1H4H, H4/H, H4FH, AND, HIST1H4I, H4/M, H4FM, AND, HIST1H4J, H4/E, H4FE, AND, HIST1H4K, H4/D, H4FD, AND, HIST1H4L, H4/K, H4FK, AND, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, AND, HIST2H4B, H4/O, H4FO, AND, HIST4H4

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 2,10E+09

Uniport ID: P62805

Modified: Epigenetics and Nuclear Signaling

species: Human

Species Reactivity: Human

Immunogen: A synthesized peptide

Tested Applications: ELISA, IHC, FC; Recommended dilution: IHC:1:50-1:500

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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