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ENO1 Monoclonal Antibody | CSB-MA007670A0m

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CSB-MA007670A0m
Availability:
3 to 7 Working Days
  • ENO1 Monoclonal Antibody
  • Western Blot<br />
 Positive WB detected in: K562 whole cell lysate, NIH/3T3 whole cell lysate, Rat Brain tissue, Mouse Brain tissue, Rabbit Skeletal Muscle tissue, Rat Kidney tissue, Rabbit Kidney tissue <br />
 All lanes ENO1 antibody at 1:10000<br />
 Secondary<br />
 Goat polyclonal to mouse IgG at 0.261ug/ml<br />
 Predicted band size: 47 KDa<br />
 Observed band size: 47 KDa<br />
 Exposure time: 1min
  • Western Blot<br />
 Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate <br />
 All lanes ENO1 antibody at 1:10000<br />
 Secondary<br />
 Goat polyclonal to mouse IgG at 0.261ug/ml<br />
 Predicted band size: 47 KDa<br />
 Observed band size: 47 KDa<br />
 Exposure time: 10s
  • Western Blot<br />
 Positive WB detected in: HepG2 whole cell lysate at 20µg, 10µg, 5µg, 2.5µg, 1.25µg, 0.625µg<br />
 All lanes: ENO1 antibody at 1:5000<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/10000 dilution<br />
 Predicted band size: 47 kDa<br />
 Observed band size: 47 KDa<br />
 Exposure time: 10s
  • Western Blot<br />
 Positive WB detected in: MCF-7 whole cell lysate<br />
 All lanes: ENO1 antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/10000 dilution<br />
 Predicted band size: 47 kDa<br />
 Observed band size: 47 KDa<br />
 Exposure time: 10s
  • IHC image of CSB-MA007670A0m diluted at 1:500 and staining in paraffin-embedded human liver cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA007670A0m diluted at 1:500 and staining in paraffin-embedded human liver cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA007670A0m diluted at 1:500 and staining in paraffin-embedded human colon cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA007670A0m diluted at 1:500 and staining in paraffin-embedded human colon cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA007670A0m diluted at 1:500 and staining in paraffin-embedded human pancreas tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA007670A0m diluted at 1:500 and staining in paraffin-embedded human pancreas tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of MCF-7 cells with CSB-MA007670A0m at 1:130, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Overlay histogram showing Hela cells stained with CSB-MA007670A0m (red line) at 1:260. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10, 000 events was performed.
  • Overlay histogram showing MCF-7 cells stained with CSB-MA007670A0m (red line) at 1:260. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10, 000 events was performed.
  • Immunoprecipitating ENO1 in HepG2 whole cell lysate<br />
 Lane 1: Mouse control IgG (1µg) instead of CSB-MA007670A0m in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000) <br />
 Lane 2: CSB-MA007670A1m (2µl) + HepG2 whole cell lysate (500µg) <br />
 Lane 3: HepG2 whole cell lysate (10µg) <br />
€373.00 - €555.00
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Description

ENO1 Monoclonal Antibody | CSB-MA007670A0m | Cusabio

ENO1 Monoclonal Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to [email protected]

Product Type: Monoclonal Antibody

Target Name: ENO1

Aliases: Alpha-enolase (2-phospho-D-glycerate hydro-lyase) (C-myc promoter-binding protein) (Enolase 1) (MBP-1) (MPB-1) (Non-neural enolase) ( NNE) (Phosphopyruvate hydratase) (Plasminogen-binding protein) , ENO1, ENO1L1, MBPB1, MPB1

Relevance: ENO1 encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.

Isotype: IgG1

Conjugate: Non-conjugated

Clone Number: 8H9G12

Uniport ID: P06733

Alternatives To SCBT: #N/A

Host Species: Mouse

Species Reactivity: Human, Mouse, Rat, Rabbit

Immunogen: Recombinant Human Alpha-enolase protein (2-434AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, FC, IP

Tested Applications: ELISA, WB, IHC, IF, FC, IP; Recommended dilution: WB: 1:5000-1:320000, IHC:1:200-1:500, IF:1:50-1:200, FC:1:100-1:300, IP:1µl-4µl

Purification Method: >95%, Protein G purified

Dilution Ratio1: WB: 1:5000-1:320000

Dilution Ratio2: IHC:1:200-1:500

Dilution Ratio3: IF:1:50-1:200

Dilution Ratio4: FC:1:100-1:300

Dilution Ratio5: IP:1µl-4µl

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Immunology

Research Areas: Immunology

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