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ENO1 Monoclonal Antibody | CSB-MA007670A1m

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SKU:
CSB-MA007670A1m
Availability:
3 to 7 Working Days
  • ENO1 Monoclonal Antibody
  • Western Blot<br />
 Positive WB detected in: K562 whole cell lysate, Rabbit Skeletal Muscle tissue, Rabbit Kidney lysate<br />
 All lanes ENO1 antibody at 1:10000<br />
 Secondary<br />
 Goat polyclonal to mouse IgG at 0.261ug/ml<br />
 Predicted band size: 47 KDa<br />
 Observed band size: 47 KDa<br />
 Exposure time: 1min
  • Western Blot<br />
 Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate <br />
 All lanes ENO1 antibody at 1:10000<br />
 Secondary<br />
 Goat polyclonal to mouse IgG at 0.261ug/ml<br />
 Predicted band size: 47 KDa<br />
 Observed band size: 47 KDa<br />
 Exposure time: 10s
  • Western Blot<br />
 Positive WB detected in: HepG2 whole cell lysate at 20µg, 10µg, 5µg, 2.5µg, 1.25µg, 0.625µg<br />
 All lanes: ENO1 antibody at 1:5000<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/10000 dilution<br />
 Predicted band size: 47 kDa<br />
 Observed band size: 47 KDa<br />
 Exposure time: 10s
  • Western Blot<br />
 Positive WB detected in: MCF-7 whole cell lysate<br />
 All lanes: ENO1 antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/10000 dilution<br />
 Predicted band size: 47 kDa<br />
 Observed band size: 47 KDa<br />
 Exposure time: 10s
  • Immunofluorescence staining of MCF-7 cells with CSB-MA007670A0m at 1:270, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Overlay histogram showing MCF-7 cells stained with CSB-MA007670A1m (red line) at 1:550. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10, 000 events was performed.
  • Overlay histogram showing Hela cells stained with CSB-MA007670A1m (red line) at 1:550. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10, 000 events was performed.
  • Immunoprecipitating ENO1 in HepG2 whole cell lysate<br />
 Lane 1: Mouse control IgG (1µg) instead of CSB-MA007670A1m in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000) <br />
 Lane 2: CSB-MA007670A1m (1µl) + HepG2 whole cell lysate (500µg) <br />
 Lane 3: HepG2 whole cell lysate (10µg) <br />
€373.00 - €555.00
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Description

ENO1 Monoclonal Antibody | CSB-MA007670A1m | Cusabio

ENO1 Monoclonal Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Monoclonal Antibody

Target Name: ENO1

Aliases: Alpha-enolase (2-phospho-D-glycerate hydro-lyase) (C-myc promoter-binding protein) (Enolase 1) (MBP-1) (MPB-1) (Non-neural enolase) ( NNE) (Phosphopyruvate hydratase) (Plasminogen-binding protein) , ENO1, ENO1L1, MBPB1, MPB1

Relevance: ENO1 encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.

Isotype: IgG1

Conjugate: Non-conjugated

Clone Number: 4D11F5

Uniport ID: P06733

Alternatives To SCBT: #N/A

Host Species: Mouse

Species Reactivity: Human, Mouse, Rat, Rabbit

Immunogen: Recombinant Human Alpha-enolase protein (2-434AA)

Immunogen Species: Human

Applications: ELISA, WB, IF, FC, IP

Tested Applications: ELISA, WB, IF, FC, IP; Recommended dilution: WB: 1:5000-1:640000, IF:1:50-1:300, FC:1:100-1:600, IP:1µl-4µl

Purification Method: >95%, Protein G purified

Dilution Ratio1: WB: 1:5000-1:640000

Dilution Ratio2:

Dilution Ratio3: IF:1:50-1:300

Dilution Ratio4: FC:1:100-1:600

Dilution Ratio5: IP:1µl-4µl

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Immunology

Research Areas: Immunology

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