ELANE Antibody | CSB-RA200985A0HU

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SKU:
CSB-RA200985A0HU
Availability:
3 to 7 Working Days
  • ELANE Antibody
  • IHC image of CSB-RA200985A0HU diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • Overlay histogram showing Jurkat cells stained with CSB-RA200985A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
€373.00 - €555.00

Description

ELANE Antibody | CSB-RA200985A0HU | Cusabio

ELANE Antibody is Available at Gentaur Genprice with the fastest delivery.

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Antibody Type: Recombinant

Target Names: ELANE

Aliases: Neutrophil elastase (EC 3.4.21.37) (Bone marrow serine protease) (Elastase-2) (Human leukocyte elastase) (HLE) (Medullasin) (PMN elastase), ELANE, ELA2

Background: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 2H12

Uniport ID: P08246

Modified: Cancer; Immunology; Microbiology; Signal transduction

species: Homo sapiens (Human)

Species Reactivity: Human

Immunogen: A synthesized peptide derived from human Neutrophil Elastase

Tested Applications: ELISA, IHC, FC; Recommended dilution: IHC:1:50-1:200, FC:1:20-1:200

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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