E-Tag Monoclonal Antibody | CSB-MA000151M0m

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SKU:
CSB-MA000151M0m
Availability:
3 to 7 Working Days
  • E-Tag Monoclonal Antibody
  • Western Blot<br />
 Positive WB detected in: E-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng<br />
 All lanes: E-Tag antibody at 1:1000<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/10000 dilution<br />
 Predicted band size: 36 kDa<br />
 Observed band size: 36 kDa<br />
  • Western Blot<br />
 Positive WB detected in: E-tagged fusion protein<br />
 All lanes: E-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/10000 dilution<br />
 Predicted band size: 36 kDa<br />
 Observed band size: 36 kDa<br />
  • Immunoprecipitating E-Tag in 293F transfected whole cell lysate<br />
 Lane 1: Mouse control IgG (1µg) instead of CSB-MA000151M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br />
 Lane 2: CSB-MA000151M0m (5µg) + 293F transfected whole cell lysate (500µg)<br />
 Lane 3: 293F transfected whole cell lysate (20µg)<br />
  • ELISA E-Tag antibody in E-tagged fusion protein and Control protein(no E-tag)<br />
 Antigen at dilution of 1ug/ml<br />
 E-Tag antibody at (0-0.15ug)<br />
 Goat polyclonal to Mouse IgG at 1/50000 dilution<br />
  • Western Blot<br />
 Positive WB detected in: 1-3 lanes: E‐tagged fusion protein, 4-5 lanes: Recombinant protein without E-tagged, 6 lane: BSA-E-Tag<br /> All lanes: E-Tag antibody at 1:1000<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/10000 dilution<br />
 Predicted band size: 30, 68, 55, 67-157kDa<br />
 Observed band size: 30, 68, 55, 67-157kDa<br />
€256.00 - €360.00

Description

E-Tag Monoclonal Antibody | CSB-MA000151M0m | Cusabio

E-Tag Monoclonal Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Tag/Control Antibodies

Target Name: E-Tag

Aliases: E-Tag

Relevance:

Isotype: IgG1

Conjugate: Non-conjugated

Clone Number: 11H12B3

Host Species: Mouse

Species Reactivity: All

Immunogen: GAPVPYPDPLEPRC synthetic peptide conjugate to KLH

Applications: ELISA, WB, IP

Tested Applications: ELISA, WB, IP; Recommended dilution: ELISA:1:4000-1:8000, WB:1:1000-1:320000, IP:1µg-5µg

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:4000-1:8000

Dilution Ratio2: WB:1:1000-1:320000

Dilution Ratio3: IP:1µg-5µg

Dilution Ratio4:

Dilution Ratio5:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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