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HA-Tag Monoclonal Antibody | CSB-MA000141M0m

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SKU:
CSB-MA000141M0m
Availability:
3 to 7 Working Days
  • HA-Tag Monoclonal Antibody
  • Western Blot<br />
 Positive WB detected in: 3 different overexpression lysates with HA tagged<br />
 All lanes: HA-Tag antibody at 1:1000<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/10000 dilution<br />
 Predicted band size: 35, 35, 48 kDa<br />
 Observed band size: 35, 35, 48 kDa</p>
  • Western Blot<br />
 Positive WB detected in: HA-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng<br />
 All lanes: HA-Tag antibody at 1:1000<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/10000 dilution<br />
 Predicted band size: 35 kDa<br />
 Observed band size: 35 kDa</p>
  • Western Blot<br />
 Positive WB detected in: HA-tagged fusion protein<br />
 All lanes: HA-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/10000 dilution<br />
 Predicted band size: 35 kDa<br />
 Observed band size: 35 kDa</p>
  • Western Blot<br />
 Positive WB detected in: 6 different recombinant proteins with HA tagged, Hela whole cell lysate, 3T3 whole cell lysate<br />
 All lanes: HA-Tag antibody at 1:1000<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/10000 dilution</p>
  • Immunofluorescence staining of 293F cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4&deg;C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
  • Immunofluorescence staining of 293F transfected cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4&deg;C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
  • Immunofluorescence staining of 293F cells and 293F transfected cells with Company A, Company B, Company C, Company D, Company E, CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4&deg;C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
  • Immunoprecipitating HA-Tag in 293F transfected whole cell lysate
 Lane 1: Mouse control IgG (1µg) instead of CSB-MA000141M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
 Lane 2: CSB-MA009476A0m (5µg) + 293F transfected whole cell lysate (500µg)
 Lane 3: 293F transfected whole cell lysate (20µg)
  • Immunoprecipitating HA-Tag in 293F transfected whole cell lysate
 Lane 1: Mouse control IgG (1µg) instead of CSB-MA000141M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
 Lane 2: Company A (5µg), Company B (5µg),Company C (5µg),Company D (5µg),Company E (5µg),CSB-MA009476A0m (5µg) + 293F transfected whole cell lysate (500µg)
 Lane 3: 293F transfected whole cell lysate (20µg)
€256.00 - €360.00
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Description

HA-Tag Monoclonal Antibody | CSB-MA000141M0m | Cusabio

HA-Tag Monoclonal Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to [email protected]

Product Type: Tag/Control Antibodies

Target Name: HA-Tag

Aliases: HA-Tag

Relevance: Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Isotype: IgG2b

Conjugate: Non-conjugated

Clone Number: 18B11H6

Host Species: Mouse

Species Reactivity: All

Immunogen: YPYDVPDYA synthetic peptide conjugate to KLH

Applications: ELISA, WB, IF, FC, IP

Tested Applications: ELISA, WB, IF, FC, IP; Recommended dilution: WB:1:5000-1:160000, IF:1:50-1:200, IP: 1:5000-1:100000, FC:1:50-1:200

Purification Method: >95%,Protein A purified

Dilution Ratio1: WB:1:5000-1:160000

Dilution Ratio2: IF:1:50-1:200

Dilution Ratio3: IP:1:5000-1:100000

Dilution Ratio4: FC:1:50-1:200

Dilution Ratio5:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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