CD146 Monoclonal Antibody | CSB-MA013563A0m

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SKU:
CSB-MA013563A0m
Availability:
3 to 7 Working Days
  • CD146 Monoclonal Antibody
  • Western Blot<br />
 Positive WB detected in: MCF-7 whole cell lysate<br />
 All lanes: CD146 antibody at 1:1250<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/50000 dilution<br />
 Predicted band size: 72, 58 kDa<br />
 Observed band size: 120 kDa<br />
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate<br />
 All lanes: CD146 antibody at 1:500<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/50000 dilution<br />
 Predicted band size: 72, 58 kDa<br />
 Observed band size: 120 kDa<br />
  • Immunofluorescence staining of A375 cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescence staining of Hela cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescence staining of MCF-7 cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescence staining of THP-1 cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Overlay histogram showing MCF-7 cells stained with CSB-MA013563A0m (red line) at 1:400. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10, 000 events was performed.
$447.60 - $666.00

Description

CD146 Monoclonal Antibody | CSB-MA013563A0m | Cusabio

CD146 Monoclonal Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Monoclonal Antibody

Target Name: CD146

Aliases: Cell surface glycoprotein MUC18 (Cell surface glycoprotein P1H12) (Melanoma cell adhesion molecule) (Melanoma-associated antigen A32) (Melanoma-associated antigen MUC18) (S-endo 1 endothelial-associated antigen) (CD antigen CD146) , MCAM, MUC18

Relevance: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

Isotype: IgG2a

Conjugate: Non-conjugated

Clone Number: 4D10C7

Uniport ID: P43121

Alternatives To SCBT: #N/A

Host Species: Mouse

Species Reactivity: Human

Immunogen: Recombinant Human Cell surface glycoprotein MUC18 protein (50-646AA)

Immunogen Species: Human

Applications: ELISA, WB, IF, FC

Tested Applications: ELISA, WB, IF, FC; Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200

Purification Method: >95%, Protein G purified

Dilution Ratio1: WB:1:500-1:2000

Dilution Ratio2: IF:1:50-1:200

Dilution Ratio3:

Dilution Ratio4:

Dilution Ratio5:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Immunology

Research Areas: Immunology

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