ATP5F1A Antibody | CSB-PA002344HA01HU

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3 to 7 Working Days
  • ATP5F1A Antibody
  • Western Blot<br />
 Positive WB detected in: K562 whole cell lysate, Mouse heart tissue<br />
 All lanes: ATP5F1A antibody at 2.5µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 60, 55, 58 kDa<br />
 Observed band size: 60 kDa<br />
  • Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA002344HA01HU at dilution of 1:100
  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA002344HA01HU at dilution of 1:100
  • Immunoprecipitating ATP5A1 in Hela whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of (1µg) instead of CSB-PA002344HA01HU in Hela whole cell lysate.
 For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) <br />
 Lane 2: CSB-PA002344HA01HU (4µg) + Hela whole cell lysate (500µg) <br />
 Lane 3: Hela whole cell lysate (20µg) <br />
£252.64 - £390.96


ATP5F1A Antibody | CSB-PA002344HA01HU | Cusabio

ATP5F1A Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: ATP5F1A

Aliases: ATP synthase subunit alpha, mitochondrial, ATP5A1, ATP5A ATP5AL2 ATPM

Background: Mitochondrial membrane ATP synthase (F (1) F (0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F (1) - containing the extramembraneous catalytic core, and F (0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F (1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F (1) . Rotation of the central stalk against the surrounding alpha (3) beta (3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P25705

Host Species: Rabbit

Species Reactivity: Human, Mouse

Immunogen: Recombinant Human ATP synthase subunit alpha, mitochondrial protein (59-257AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IP

Tested Applications: ELISA, WB, IHC, IP; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

Dilution Ratio3: IHC:1:20-1:200

Dilution Ratio4: IP:1:200-1:2000

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Tags & Cell Markers

Research Areas: Tags & Cell Markers;Metabolism;Signal transduction

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