ATP5F1A Antibody | CSB-PA002344HA01HU
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ATP5F1A Antibody | CSB-PA002344HA01HU | Cusabio
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Product Type: Polyclonal Antibody
Target Names: ATP5F1A
Aliases: ATP synthase subunit alpha, mitochondrial, ATP5A1, ATP5A ATP5AL2 ATPM
Background: Mitochondrial membrane ATP synthase (F (1) F (0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F (1) - containing the extramembraneous catalytic core, and F (0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F (1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F (1) . Rotation of the central stalk against the surrounding alpha (3) beta (3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity) .
Uniport ID: P25705
Host Species: Rabbit
Species Reactivity: Human, Mouse
Immunogen: Recombinant Human ATP synthase subunit alpha, mitochondrial protein (59-257AA)
Immunogen Species: Human
Applications: ELISA, WB, IHC, IP
Tested Applications: ELISA, WB, IHC, IP; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000
Purification Method: >95%, Protein G purified
Dilution Ratio1: ELISA:1:2000-1:10000
Dilution Ratio2: WB:1:1000-1:5000
Dilution Ratio3: IHC:1:20-1:200
Dilution Ratio4: IP:1:200-1:2000
Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Initial Research Areas: Tags & Cell Markers
Research Areas: Tags & Cell Markers;Metabolism;Signal transduction