YWHAZ Monoclonal Antibody | CSB-MA026293A0m

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SKU:
CSB-MA026293A0m
Availability:
3 to 7 Working Days
  • YWHAZ Monoclonal Antibody
  • Western Blot<br /> Positive WB detected in: NIH/3T3 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, RAW264.7 whole cell lysate, Rat Brain tissue <br /> All lanes YWHAZ antibody at 1:5000<br /> Secondary<br /> Goat polyclonal to mouse IgG at 1/50000 dilution<br /> Predicted band size: 34 KDa<br /> Observed band size: 34 KDa<br /> Exposure time：1min
  • Western Blot<br /> Positive WB detected in: Hela whole cell lysate at 20μg, 10μg, 5μg, 2.5μg, 1.25μg, 0.625μg All lanes: YWHAZ antibody at 1:5000<br /> Secondary<br /> Goat polyclonal to mouse IgG at 1/50000 dilution<br /> Predicted band size: 34 KDa<br /> Observed band size: 34 KDa<br /> Exposure time：5min
  • IHC image of CSB-MA026293A0m diluted at 1:200 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at 37℃. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
  • IHC image of CSB-MA026293A0m diluted at 1:200 and staining in paraffin-embedded human liver cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at 37℃. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
  • IHC image of CSB-MA026293A0m diluted at 1:200 and staining in paraffin-embedded human breast cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at 37℃. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
  • Immunofluorescence staining of A549 cells with CSB-MA026293A0m at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescence staining of Hela cells with CSB-MA026293A0m at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescence staining of U251 cells with CSB-MA026293A0m at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Overlay histogram showing A549 cells stained with CSB-MA026293A0m (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG (H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • Overlay histogram showing Hela cells stained with CSB-MA026293A0m (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG (H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • Overlay histogram showing HepG2 cells stained with CSB-MA026293A0m (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG (H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
$447.60 - $666.00

Description

YWHAZ Monoclonal Antibody | CSB-MA026293A0m | Cusabio

YWHAZ Monoclonal Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Monoclonal Antibody

Target Name: YWHAZ

Aliases: 14 3 3 delta antibody; 14 3 3 protein zeta/delta antibody; 14 3 3 protein/cytosolic phospholipase A2 antibody; 14 3 3 zeta antibody; 14-3-3 protein zeta/delta antibody; 1433Z_HUMAN antibody; Epididymis luminal protein 4 antibody; Epididymis secretory protein Li 3 antibody; HEL S 3 antibody; HEL4 antibody; KCIP-1 antibody; KCIP1 antibody; MGC111427 antibody; MGC126532 antibody; MGC138156 antibody; Phospholipase A2 antibody; Protein kinase C inhibitor protein 1 antibody; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; delta polypeptide antibody; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; zeta antibody; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; zeta polypeptide antibody; Tyrosine 3/tryptophan 5 monooxygenase activation protein; zeta polypeptide antibody; YWHAD antibody; YWHAZ antibody

Relevance:

Isotype: IgG2b

Conjugate: Non-conjugated

Clone Number: 19G7E9

Uniport ID: P63104

Alternatives To SCBT:

Host Species: Mouse

Species Reactivity: Human, Mouse, Rat

Immunogen: Recombinant Human 14-3-3 protein zeta/delta protein (133-212AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, FC

Tested Applications: ELISA, WB, IHC, IF, FC; Recommended dilution: WB：1:5000-160000, IHC：1:200, IF：1:50, FC：1:100

Purification Method: >95%, Protein A purified

Dilution Ratio1: WB：1:5000-160000

Dilution Ratio2: IHC:1:50-1:200

Dilution Ratio3: IF:1:50-1:200

Dilution Ratio4: FC:1:50-1:200

Dilution Ratio5:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas:

Research Areas:

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