WDR59 Antibody | CSB-PA764810LA01HU

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SKU:
CSB-PA764810LA01HU
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  • WDR59 Antibody
  • Immunofluorescence staining of U251 cells with CSB-PA764810LA01HU at 1:173, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
  • Western Blot<br />
 Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate<br />
 All lanes: WDR59 antibody at 1:2000<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 110, 65, 63, 47 kDa<br />
 Observed band size: 110 kDa<br />
£252.64 - £390.96

Description

WDR59 Antibody | CSB-PA764810LA01HU | Cusabio

WDR59 Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: WDR59

Aliases: GATOR complex protein WDR59 (WD repeat-containing protein 59), WDR59, KIAA1923

Background: As a component of the GATOR subcomplex GATOR2, functions within the amino acid-sensing branch of the TORC1 signaling pathway. Indirectly activates mTORC1 and the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex (PubMed:23723238) . It is negatively regulated by the upstream amino acid sensors SESN2 and CASTOR1 (PubMed:25457612, PubMed:27487210) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q6PJI9

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human GATOR complex protein WDR59 protein (619-862AA)

Immunogen Species: Homo sapiens (Human)

Applications: ELISA, WB, IF

Tested Applications: ELISA, WB, IF; Recommended dilution: WB:1:1000-1:5000, IF:1:50-1:200

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

Dilution Ratio3: IF:1:50-1:200

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Cell Biology

Research Areas: Cell biology

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