TP53 (Ab-6) Antibody | CSB-PA277007

TP53 (Ab-6) Antibody | CSB-PA277007 | Cusabio

TP53 (Ab-6) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: TP53

Aliases: Tumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13; TP53;

Background:

p53 is a nuclear protein which plays an essential role in the regulation of cell cycle specifically in the transition from G0 to G1. It is found in very low levels in normal cells however in a variety of transformed cell lines in high amounts and believed to contribute to transformation and malignancy. The open reading frame of p53 is 393 amino acids long, with the central region (consisting of amino acids from about 100 to 300) containing the DNA-binding domain. This proteolysis-resistant core is flanked by a C-terminal end mediating oligomerization and an N-terminal end containing a strong transcription activation signal. p53 binds as a tetramer to a PBS (p53-Binding Site) and activates the expression of downstream genes that inhibit growth and/or invasion. p53 binds as a tetramer to a p53-binding site (PBS) and to activate the expression of adjacent genes that inhibit growth and/or invasion. Deletion of one or both p53 alleles reduces the expression of tetramers, resulting in decreased expression of the growth inhibitory genes

Lin T, et al. (2005) Nat Cell Biol; 7 (2) : 165-71.
Vega FM, et al. (2004) Mol Cell Biol; 24 (23) : 10366-80.
Li J, et al. (2004) J Biol Chem; 279 (40) : 41275-9.
Wang J, et al. (2004) J Biol Chem; 279 (38) : 39584-92.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P04637

Host Species: Rabbit

Species Reactivity: Human, Mouse

Immunogen: Peptide sequence around aa.4~8 (P-Q-S-D-P) derived from Human p53.

Immunogen Species: Human

Applications: ELISA, WB

Tested Applications: ELISA, WB;WB:1:500-1:1000

Purification Method: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:1000

Dilution Ratio3:

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Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Cell Biology

Research Areas: Epigenetics & Nuclear Signaling;Cancer;Cell biology