TNNT2 Antibody | CSB-PA10047A0Rb

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CSB-PA10047A0Rb
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3 to 7 Working Days
  • TNNT2 Antibody
  • Western Blot<br />
 Positive WB detected in: Mouse skeletal muscle tissue<br />
 All lanes: TNNT2 antibody at 4µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 36, 35, 32, 31 kDa<br />
 Observed band size: 36, 32 kDa<br />
  • IHC image of CSB-PA10047A0Rb diluted at 1:200 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of Hela cells with CSB-PA10047A0Rb at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
€315.80 - €488.70

Description

TNNT2 Antibody | CSB-PA10047A0Rb | Cusabio

TNNT2 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: TNNT2

Aliases: Troponin T, cardiac muscle (TnTc) (Cardiac muscle troponin T) (cTnT), TNNT2

Background: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P45379

Host Species: Rabbit

Species Reactivity: Human, Mouse

Immunogen: Recombinant Human Troponin T, cardiac muscle protein (138-298AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

Dilution Ratio3: IHC:1:20-1:200

Dilution Ratio4: IF:1:50-1:200

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Cardiovascular;Signal transduction;Stem cells

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