TNFRSF10A Antibody | CSB-PA023964LA01HU

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CSB-PA023964LA01HU
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3 to 7 Working Days
  • TNFRSF10A Antibody
  • Western Blot<br />
 Positive WB detected in: HEK293 whole cell lysate<br />
 All lanes: TNFRSF10A antibody at 3.2µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 51 kDa<br />
 Observed band size: 51 kDa<br />
  • IHC image of CSB-PA023964LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-PA023964LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescent analysis of A549 cells using CSB-PA023964LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L)
  • Immunoprecipitating TNFRSF10A in 293 whole cell lysate <br />
 Lane 1: Rabbit control IgG (1µg) instead of CSB-PA023964LA01HU in 293 whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA023964LA01HU (6µg) + 293 whole cell lysate (500µg) <br />
 Lane 3: 293 whole cell lysate (10µg) <br />
£252.64 - £390.96

Description

TNFRSF10A Antibody | CSB-PA023964LA01HU | Cusabio

TNFRSF10A Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: TNFRSF10A

Aliases: Tumor necrosis factor receptor superfamily member 10A (Death receptor 4) (TNF-related apoptosis-inducing ligand receptor 1) (TRAIL receptor 1) (TRAIL-R1) (CD antigen CD261), TNFRSF10A, APO2 DR4 TRAILR1

Background: Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: O00220

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Tumor necrosis factor receptor superfamily member 10A protein (263-468AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, IP

Tested Applications: ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:1000-1:5000, IHC:1:100-1:500, IF:1:50-1:500, IP:1:200-1:2000

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

Dilution Ratio3: IHC:1:100-1:500

Dilution Ratio4: IF:1:50-1:500

Dilution Ratio5: IP:1:200-1:2000

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Cell Biology

Research Areas: Cardiovascular;Cell biology;Immunology

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