SYNJ1 Antibody | CSB-PA023016LA01HU

(No reviews yet) Write a Review
SKU:
CSB-PA023016LA01HU
Availability:
3 to 7 Working Days
  • SYNJ1 Antibody
  • IHC image of CSB-PA023016LA01HU diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of NIH/3T3 cells with CSB-PA023016LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
  • Western Blot<br />
 Positive WB detected in: HT29 whole cell lysate<br />
 All lanes: SYNJ1 antibody at 3.7µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 174, 145, 144, 59 kDa<br />
 Observed band size: 174 kDa<br />
£252.64 - £390.96

Description

SYNJ1 Antibody | CSB-PA023016LA01HU | Cusabio

SYNJ1 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: SYNJ1

Aliases: Synaptojanin-1 (EC 3.1.3.36) (Synaptic inositol 1, 4, 5-trisphosphate 5-phosphatase 1), SYNJ1, KIAA0910

Background: Phosphatase that acts on various phosphoinositides, including phosphatidylinositol 4-phosphate, phosphatidylinositol (4, 5) -bisphosphate and phosphatidylinositol (3, 4, 5) -trisphosphate (PubMed:27435091) . Has a role in clathrin-mediated endocytosis (By similarity) . Hydrolyzes PIP2 bound to actin regulatory proteins resulting in the rearrangement of actin filaments downstream of tyrosine kinase and ASH/GRB2 (By similarity) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: O43426

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Synaptojanin-1 protein (1030-1261AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:5000

Dilution Ratio3: IHC:1:200-1:500

Dilution Ratio4: IF:1:50-1:200

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Neuroscience

Research Areas: Neuroscience

View AllClose

0 Reviews

View AllClose