SUZ12 Antibody | CSB-RA159561A0HU

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SKU:
CSB-RA159561A0HU
Availability:
3 to 7 Working Days
  • SUZ12 Antibody
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, HL60 whole cell lysate, THP-1 whole cell lysate, K562 whole cell lysate<br />
 All lanes: SUZ12 antibody at 1:2000<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 84 kDa<br />
 Observed band size: 90 kDa<br />
  • IHC image of CSB-RA159561A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • Immunoprecipitating SUZ12 in K562 whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-RA159561A0HU in K562 whole cell lysate.
 For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br />
 Lane 2: CSB-RA159561A0HU(2µg)+ K562 whole cell lysate(500µg)<br />
 Lane 3: K562 whole cell lysate (10µg)<br />
£298.40 - £444.00

Description

SUZ12 Antibody | CSB-RA159561A0HU | Cusabio

SUZ12 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Antibody Type: Recombinant

Target Names: SUZ12

Aliases: Polycomb protein SUZ12 (Chromatin precipitated E2F target 9 protein) (ChET 9 protein) (Joined to JAZF1 protein) (Suppressor of zeste 12 protein homolog), SUZ12, CHET9 JJAZ1 KIAA0160

Background: Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 1B10

Uniport ID: Q15022

Modified: Epigenetics and Nuclear Signaling

species: Homo sapiens (Human)

Species Reactivity: Human

Immunogen: A synthesized peptide derived from human SUZ12

Tested Applications: ELISA, WB, IHC, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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