SOD2 Antibody | CSB-PA022398LA01HU

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SKU:
CSB-PA022398LA01HU
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  • SOD2 Antibody
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Rat brain tissue, Rat heart tissue, Mouse brain tissu<br />
 All lanes: SOD2 antibody at 3.4µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 25, 21, 19, 20 kDa<br />
 Observed band size: 25 kDa<br />
  • IHC image of CSB-PA022398LA01HU diluted at 1:560 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of Hela cells with CSB-PA022398LA01HU at 1:250, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
  • Immunoprecipitating SOD2 in K562 whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-PA022398LA01HU in K562 whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA022398LA01HU (8µg) + K562 whole cell lysate (500µg) <br />
 Lane 3: K562 whole cell lysate (10µg) <br />
$378.96 - $586.44

Description

SOD2 Antibody | CSB-PA022398LA01HU | Cusabio

SOD2 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: SOD2

Aliases: Superoxide dismutase [Mn], mitochondrial (EC 1.15.1.1), SOD2

Background: Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P04179

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Immunogen: Recombinant Human Superoxide dismutase [Mn], mitochondrial protein (64-175AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, IP

Tested Applications: ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500, IP:1:200-1:2000

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:5000

Dilution Ratio3: IHC:1:500-1:1000

Dilution Ratio4: IF:1:200-1:500

Dilution Ratio5: IP:1:200-1:2000

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Cell Biology

Research Areas: Neuroscience;Cancer;Cardiovascular;Cell biology;Metabolism;Signal transduction

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