SLC25A24 Antibody | CSB-PA743993LA01HU

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SKU:
CSB-PA743993LA01HU
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  • SLC25A24 Antibody
  • Western Blot<br />
 Positive WB detected in: 293T whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, Rat kidney tissue, Mouse heart tissue<br />
 All lanes: SLC25A24 antibody at 5µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 54, 52 kDa<br />
 Observed band size: 54 kDa<br />
  • IHC image of CSB-PA743993LA01HU diluted at 1:250 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of U251 cells with CSB-PA743993LA01HU at 1:110, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
  • Western Blot<br />
 Positive WB detected in: 293T whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, U87 whole cell lysate, K562 whole cell lysate<br />
 All lanes: SLC25A24 antibody at 1:1500<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 54, 52 kDa<br />
 Observed band size: 54 kDa<br />
£252.64 - £390.96

Description

SLC25A24 Antibody | CSB-PA743993LA01HU | Cusabio

SLC25A24 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: SLC25A24

Aliases: Calcium-binding mitochondrial carrier protein SCaMC-1 (Mitochondrial ATP-Mg/Pi carrier protein 1) (Mitochondrial Ca (2+) -dependent solute carrier protein 1) (Small calcium-binding mitochondrial carrier protein 1) (Solute carrier family 25 member 24), SLC25A24, APC1 MCSC1 SCAMC1

Background: Calcium-dependent mitochondrial solute carrier. Mediates the reversible, electroneutral exchange of Mg-ATP or Mg-ADP against phosphate ions, catalyzing the net uptake or efflux of adenine nucleotides across the mitochondrial inner membrane. Nucleotide transport is inactive when cytosolic calcium levels are low, and is activated by an increase in cytosolic calcium levels. May play a role in protecting cells against oxidative stress-induced cell death, probably by promoting the formation of calcium-phosphate precipitates in the mitochondrial matrix, and thereby buffering calcium levels in the mitochondrial matrix.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q6NUK1

Host Species: Rabbit

Species Reactivity: Human, Rat, Mouse

Immunogen: Recombinant Human Calcium-binding mitochondrial carrier protein SCaMC-1 protein (1-197AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:5000

Dilution Ratio3: IHC:1:200-1:500

Dilution Ratio4: IF:1:50-1:200

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Metabolism;Signal transduction

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