SAE1 Antibody | CSB-RA987582A0HU

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SKU:
CSB-RA987582A0HU
Availability:
3 to 7 Working Days
  • SAE1 Antibody
  • Western Blot<br />
 Positive WB detected in: K562 whole cell lysate<br />
 All lanes: SAE1 Antibody at 1:1000<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 39, 30, 34 kDa<br />
 Observed band size: 39 kDa<br />
  • Immunofluorescence staining of Hela Cells with CSB-RA987582A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
£298.40 - £444.00

Description

SAE1 Antibody | CSB-RA987582A0HU | Cusabio

SAE1 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Antibody Type: Recombinant

Target Names: SAE1

Aliases: SUMO-activating enzyme subunit 1 (Ubiquitin-like 1-activating enzyme E1A) [Cleaved into: SUMO-activating enzyme subunit 1, N-terminally processed], SAE1, AOS1 SUA1 UBLE1A

Background: The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 9D8

Uniport ID: Q9UBE0

Modified: Cell biology

species: Homo sapiens (Human)

Species Reactivity: Human

Immunogen: A synthesized peptide derived from human SAE1

Tested Applications: ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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