RTN4RL2 Antibody | CSB-PA774805NA01HU

(No reviews yet) Write a Review
SKU:
CSB-PA774805NA01HU
Availability:
3 to 7 Working Days
  • RTN4RL2 Antibody
  • Western Blot<br />
 Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate, Hela whole cell lysate, HL-60 whole cell lysate<br />
 All lanes: RTN4RL2 antibody at 1:2000<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 47, 22 kDa<br />
 Observed band size: 47 kDa<br />
  • IHC image of CSB-PA774805NA01HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of MCF-7 cells with CSB-PA774805NA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
€315.80 - €488.70

Description

RTN4RL2 Antibody | CSB-PA774805NA01HU | Cusabio

RTN4RL2 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: RTN4RL2

Aliases: Reticulon-4 receptor-like 2 (Nogo receptor-like 3) (Nogo-66 receptor homolog 1) (Nogo-66 receptor-related protein 2) (NgR2), RTN4RL2, NGRH1 NGRL3

Background: Cell surface receptor that plays a functionally redundant role in the inhibition of neurite outgrowth mediated by MAG (By similarity) . Plays a functionally redundant role in postnatal brain development. Contributes to normal axon migration across the brain midline and normal formation of the corpus callosum. Does not seem to play a significant role in regulating axon regeneration in the adult central nervous system. Protects motoneurons against apoptosis; protection against apoptosis is probably mediated by MAG (By similarity) . Like other family members, plays a role in restricting the number dendritic spines and the number of synapses that are formed during brain development (PubMed: 22325200) . Signaling mediates activation of Rho and downstream reorganization of the actin cytoskeleton (PubMed: 22325200) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q86UN3

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence from Human Reticulon-4 receptor-like 2 protein (355-372AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

Dilution Ratio3: IHC:1:20-1:200

Dilution Ratio4: IF:1:50-1:200

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Neuroscience

Research Areas: Neuroscience

View AllClose

0 Reviews

View AllClose