RPA2 Antibody | CSB-PA01555A0Rb

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SKU:
CSB-PA01555A0Rb
Availability:
3 to 7 Working Days
  • RPA2 Antibody
  • Western Blot<br />
 Positive WB detected in: 293 whole cell lysate<br />
 All lanes: RPA2 antibody at 2µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 30, 31, 39 kDa<br />
 Observed band size: 30 kDa<br />
  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA01555A0Rb at dilution of 1:100
  • Immunofluorescent analysis of Hela cells using CSB-PA01555A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L)
  • Immunoprecipitating RPA2 in Hela whole cell lysate<br />
 Lane 1: Rabbit control IgG (1µg) instead of CSB-PA01555A0Rb in Hela whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA01555A0Rb (6µg) + Hela whole cell lysate (500µg) <br />
 Lane 3: Hela whole cell lysate (10µg) <br />
  • Western Blot<br />
 Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, HT29 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate<br />
 All lanes: RPA2 antibody at 2µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 30, 31, 39 kDa<br />
 Observed band size: 30 kDa<br />
£252.64 - £390.96

Description

RPA2 Antibody | CSB-PA01555A0Rb | Cusabio

RPA2 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: RPA2

Aliases: Replication protein A 32 kDa subunit (RP-A p32) (Replication factor A protein 2) (RF-A protein 2) (Replication protein A 34 kDa subunit) (RP-A p34), RPA2, REPA2 RPA32 RPA34

Background: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA) . aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P15927

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Replication protein A 32 kDa subunit protein (1-267AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, IP

Tested Applications: ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

Dilution Ratio3: IHC:1:20-1:200

Dilution Ratio4: IF:1:50-1:200

Dilution Ratio5: IP:1:200-1:2000

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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