RIPK1 Antibody | CSB-PA618785LA01HU

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SKU:
CSB-PA618785LA01HU
Availability:
3 to 7 Working Days
  • RIPK1 Antibody
  • Western Blot<br />
 Positive WB detected in: NIH/3T3 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, Rat liver tissue, Mouse heart tissue, Mouse kidney tissue<br />
 All lanes: RIPK1 antibody at 3.2µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 76, 71 kDa<br />
 Observed band size: 76 kDa<br />
  • Immunofluorescence staining of HepG2 cells with CSB-PA618785LA01HU at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
$378.96 - $586.44

Description

RIPK1 Antibody | CSB-PA618785LA01HU | Cusabio

RIPK1 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: RIPK1

Aliases: Receptor-interacting serine/threonine-protein kinase 1 (EC 2.7.11.1) (Cell death protein RIP) (Receptor-interacting protein 1) (RIP-1) (Serine/threonine-protein kinase RIP), RIPK1, RIP RIP1

Background: Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q13546

Host Species: Rabbit

Species Reactivity: Human, Rat, Mouse

Immunogen: Recombinant Human Receptor-interacting serine/threonine-protein kinase 1 protein (328-458AA)

Immunogen Species: Human

Applications: ELISA, WB, IF

Tested Applications: ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:200-1:500

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:5000

Dilution Ratio3: IF:1:200-1:500

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Cell Biology

Research Areas: Cancer;Cell biology;Signal transduction

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