Recombinant Yellow fever virus Genome polyprotein, partial | CSB-EP365905YAC1

Recombinant Yellow fever virus Genome polyprotein, partial | CSB-EP365905YAC1 | Cusabio

Alternative Name(s): Core protein Matrix protein NS2A NS1 NS2A-alpha Flavivirin protease NS2B regulatory subunit Non-structural protein 2B Flavivirin protease NS3 catalytic subunit Non-structural protein 3 NS4A NS4B Non-structural protein 5

Gene Names: N/A

Research Areas: Others

Organism: Yellow fever virus (strain 17D vaccine) (YFV)

AA Sequence: AHCIGITDRDFIEGVHGGTWVSATLEQDKCVTVMAPDKPSLDISLETVAIDRPAEVRKVCYNAVLTHVKINDKCPSTGEAHLAEENEGDNACKRTYSDRGWGNGCGLFGKGSIVACAKFTCAKSMSLFEVDQTKIQYVIRAQLHVGAKQENWNTDIKTLKFDALSGSQEVEFIGYGKATLECQVQTAVDFGNSYIAEMETESWIVDRQWAQDLTLPWQSGSGGVWREMHHLVEFEPPHAATIRVLALGNQEGSLKTALTGAMRVTKDTNDNNLYKLHGGHVSCRVKLSALTLKGTSYKICTDKMFFVKNPTDTGHGTVVMQVKVSKGAPCRIPVIVADDLTAAINKGILVTVNPIASTNDDEVLIEVNPPFGDSYIIVGRGDSRLTYQWHKEGSSIGKLFTQTMKGVERLAVMGDTAWDFSSAGGFFTSVGKGIHTVFGSAFQGL

Source: E.coli

Tag Info: N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged

Expression Region: 286-730aa

Sequence Info: Partial

MW: 78.4 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Capsid protein C: Plays a role in virus budding by binding to the cell membrane and gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. During virus entry, may induce genome penetration into the host cytoplasm after hemifusion induced by the surface proteins. Can migrate to the cell nucleus where it modulates host functions.

Reference: "Yellow fever 17D vaccine virus isolated from healthy vaccinees accumulates very few mutations." Xie H., Cass A.R., Barrett A.D.T. Virus Res. 55:93-99(1998)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Capsid protein C

Involvement in disease:

Subcellular Location: Capsid protein C: Virion, Host nucleus, Host cytoplasm, host perinuclear region, Host cytoplasm, SUBCELLULAR LOCATION: Peptide pr: Secreted, SUBCELLULAR LOCATION: Small envelope protein M: Virion membrane, Multi-pass membrane protein, Host endoplasmic reticulum membrane, Multi-pass membrane protein, Note=ER membrane retention is mediated by the transmembrane domains, SUBCELLULAR LOCATION: Envelope protein E: Virion membrane, Multi-pass membrane protein, Host endoplasmic reticulum membrane, Multi-pass membrane protein, Note=ER membrane retention is mediated by the transmembrane domains, SUBCELLULAR LOCATION: Non-structural protein 1: Secreted, Host endoplasmic reticulum membrane, Peripheral membrane protein, Lumenal side, Note=Located in RE-derived vesicles hosting the replication complex, SUBCELLULAR LOCATION: Non-structural protein 2A: Host endoplasmic reticulum membrane, Multi-pass membrane protein, SUBCELLULAR LOCATION: Serine protease subunit NS2B: Host endoplasmic reticulum membrane, Multi-pass membrane protein, SUBCELLULAR LOCATION: Serine protease NS3: Host endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side, Note=Remains non-covalently associated to serine protease subunit NS2B, SUBCELLULAR LOCATION: Non-structural protein 4A: Host endoplasmic reticulum membrane, Multi-pass membrane protein, Note=Located in RE-associated vesicles hosting the replication complex, SUBCELLULAR LOCATION: Non-structural protein 4B: Host endoplasmic reticulum membrane, Multi-pass membrane protein, Note=Located in RE-derived vesicles hosting the replication complex, SUBCELLULAR LOCATION: RNA-directed RNA polymerase NS5: Host endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side, Host nucleus

Protein Families: Class I-like SAM-binding methyltransferase superfamily, mRNA cap 0-1 NS5-type methyltransferase family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P03314

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: N/A

OMIM Database Link: N/A