Recombinant Vesicular stomatitis Indiana virus Glycoprotein G (G), partial | CSB-EP314306VBH
- 3 - 7 Working Days
Recombinant Vesicular stomatitis Indiana virus Glycoprotein G (G), partial | CSB-EP314306VBH | Cusabio
Alternative Name(s): G; Glycoprotein G; Fragment
Gene Names: G
Research Areas: Signal Transduction
Organism: Vesicular stomatitis Indiana virus (strain Mudd-Summers) (VSIV)
AA Sequence: KFTIVFPHNQKGNWKNVPSNYHYCPSSSDLNWHNDLIGTAIQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITQSIRSFTPSVEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQFINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFRSNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQTSVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLKYFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFPLYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESL
Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged
Expression Region: 1-423aa
Sequence Info: Partial
MW: 54.8 kDa
Purity: Greater than 85% as determined by SDS-PAGE.
Relevance: Attaches the virus to host cellular receptor, inducing clathrin-dependent endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and endosomal membrane.
Reference: "Structural basis for the recognition of LDL-receptor family members by VSV glycoprotein." Nikolic J., Belot L., Raux H., Legrand P., Gaudin Y., A Albertini A. Nat Commun 9:1029-1029(2018)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Attaches the virus to host cellular receptor, inducing clathrin-dependent endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and endosomal membrane (By similarity).
Involvement in disease:
Subcellular Location: Virion membrane, Single-pass type I membrane protein
Protein Families: Vesiculovirus glycoprotein family
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P0C2X0
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: N/A
STRING Database Link: N/A
OMIM Database Link: N/A