Recombinant Vaccinia virus Protein K3 (K3L) | CSB-EP321831VAA
- 13 - 23 Working Days
Recombinant Vaccinia virus Protein K3 (K3L) | CSB-EP321831VAA | Cusabio
Alternative Name(s): K3L; Protein K3
Gene Names: K3L
Research Areas: Others
Organism: Vaccinia virus (strain Copenhagen) (VACV)
AA Sequence: MLAFCYSLPNAGDVIKGRVYEKDYALYIYLFDYPHSEAILAESVKMHMDRYVEYRDKLVGKTVKVKVIRVDYTKGYIDVNYKRMCRHQ
Tag Info: N-terminal 6xHis-SUMO-tagged
Expression Region: 1-88aa
Sequence Info: Full Length
MW: 26.5 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Viral mimic of eIF-2-alpha that acts as a pseudosubstrate for EIF2AK2/PKR kinase. Inhibits therefore eIF-2-alpha phosphorylation by host EIF2AK2/PKR kinase and prevents protein synthesis shutoff
Reference: "Vaccinia virus-encoded eIF-2 alpha homolog abrogates the antiviral effect of interferon."Beattie E., Tartaglia J., Paoletti E.Virology 183:419-422(1991)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Viral mimic of eIF-2-alpha that acts as a pseudosubstrate for EIF2AK2/PKR kinase. Inhibits therefore eIF-2-alpha phosphorylation by host EIF2AK2/PKR kinase and prevents protein synthesis shutoff (By similarity).
Involvement in disease:
Protein Families: Poxviridae K3 protein family
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P20639
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: N/A
STRING Database Link: N/A
OMIM Database Link: N/A