Recombinant Vaccinia virus Cell surface-binding protein (D8L) | CSB-EP322653VAA
- 13 - 23 Working Days
Recombinant Vaccinia virus Cell surface-binding protein (D8L) | CSB-EP322653VAA | Cusabio
Alternative Name(s): Carbonic anhydrase homolog
Gene Names: D8L
Research Areas: Microbiology
Organism: Vaccinia virus (strain Copenhagen) (VACV)
AA Sequence: MPQQLSPINIETKKAISNARLKPLDIHYNESKPTTIQNTGKLVRINFKGGYISGGFLPNEYVLSSLHIYWGKEDDYGSNHLIDVYKYSGEINLVHWNKKKYSSYEEAKKHDDGLIIISIFLQVSDHKNVYFQKIVNQLDSIRSANTSAPFDSVFYLDNLLPSTLDYFTYLGTTIKHSADAVWIIFPTPINIHSDQLSKFRTLLSSSNHDGKPYYITENYRNPYKLNDDTQVYYSGEIIRAATTSPARENYFMRWLSDLRETCFSYYQKYIEGNKTFAIIAIVFVFILTAILFLMSRRYSREKQN
Tag Info: N-terminal 6xHis-SUMO-tagged
Expression Region: 1-304aa
Sequence Info: Full Length
MW: 51.3 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Binds to chondroitin sulfate on the cell surface to provide virion attachment to target cell.
Reference: "Vaccinia virus envelope D8L protein binds to cell surface chondroitin sulfate and mediates the adsorption of intracellular mature virions to cells."Hsiao J.C., Chung C.S., Chang W.J. Virol. 73:8750-8761(1999).
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Binds to chondroitin sulfate on the cell surface to provide virion attachment to target cell.
Involvement in disease:
Subcellular Location: Virion membrane
Protein Families: Alpha-carbonic anhydrase family
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P20508
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: N/A
STRING Database Link: N/A
OMIM Database Link: N/A