Recombinant Synechocystis sp.Ribonuclease J (rnj) | CSB-EP344938SSQ(A4)

Recombinant Synechocystis sp.Ribonuclease J (rnj) | CSB-EP344938SSQ(A4) | Cusabio

Alternative Name(s): rnj; slr0551; Ribonuclease J; RNase J; EC 3.1.-.-

Gene Names: rnj

Research Areas: Others

Organism: Synechocystis sp. (strain PCC 6803 / Kazusa)

AA Sequence: MAKNTQTQALKILPLGGLHEIGKNTCVFEYDDEILLLDAGLAFPTDDMHGVNVVLPDMTYLRENREKIKGMVVTHGHEDHIGGIAYHLKQFDIPIIYGPRLAMALLRDKLEEAGMLERTNLQTVSPREMVRLGKSFVVEFIRNTHSIADSYCLAIHTPLGVVMHSGDFKIDHTPIDGEFFDLQKVAEYGEKGVLCLLSDSTNAEVPGITPSEASVIPNLDRVFSQAEGRLMVTTFASSVHRVNIILSLAQKHQRKVAVVGRSMLNVIAHARKLGYIKCPDNLFVPLKAARNLPDQQQLILTTGSQGEPLAAMTRISNGEHPQIKIRQGDTVVFSANPIPGNTIAVVNTIDRLMMQGANVIYGKHQGIHVSGHASQEEHKMLLALTRPKFFVPVHGEHRMLVKHSQMAQAQGIPSENIVIVNNGDVIELTGDRIRVAGQVPSGIELVDQAGIVHESTMAERQQMAEDGLVTVAAALSKTGTLLAYPEVHCRGVVMTIQPKLLEELIVRTIENFLTERWSEFTHGSNGSTEVSWNALQKELESSLQRLIKRELQSSPMVLLMLQTDTPIELDQVPQNVSTVSATSATPAPRKKVVLTKTPEPKVKAKPEKKVVTTAEPSAQPVSTTKVYRRSRKRSTTSVSS

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 1-640aa

Sequence Info: Full Length

MW: 86.4 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: An RNase that has 5'-3' exonuclease and possibly endoonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay

Reference: "A large-scale protein protein interaction analysis in Synechocystis sp. PCC6803." Sato S., Shimoda Y., Muraki A., Kohara M., Nakamura Y., Tabata S. DNA Res. 14:207-216(2007)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: An RNase that has 5'-3' exonuclease and possibly endoonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay (By similarity).

Involvement in disease:

Subcellular Location: Cytoplasm

Protein Families: Metallo-beta-lactamase superfamily, RNA-metabolizing metallo-beta-lactamase-like family, Bacterial RNase J subfamily

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P54123

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A