Recombinant Symbiobacterium thermophilum DNA mismatch repair protein MutS (mutS), partial | CSB-EP727813SRW

Recombinant Symbiobacterium thermophilum DNA mismatch repair protein MutS (mutS), partial | CSB-EP727813SRW | Cusabio

Alternative Name(s): mutS; STH1757; DNA mismatch repair protein MutS

Gene Names: mutS

Research Areas: Others

Organism: Symbiobacterium thermophilum (strain T / IAM 14863)

AA Sequence: YGYCRPLVDGSTVLELKGSRHPVLERVMEEGAFVPNDLLVDTGENRVLLITGPNMGGKSTVMRQAALAVILAQAGSFVPAESAHIGLVDRVFTRVGASDDLATGRSTFMVEMTEVANILHSATERSLVVLDEVGRGTATFDGLSIAWAITEHIHQAIGCRTLFATHYHELCELEGILPGVKNYSVAVMEKGEDIIFLRKLVRGGADRS

Source: E.coli

Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged

Expression Region: 574-781aa

Sequence Info: Partial

MW: 29.4 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity

Reference: "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.Nature 392:353-358(1998)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.

Involvement in disease:

Subcellular Location:

Protein Families: DNA mismatch repair MutS family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q67NK1

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A