Recombinant Streptomyces clavuligerus Beta-lactamase inhibitory protein | CSB-EP327444FOA

Recombinant Streptomyces clavuligerus Beta-lactamase inhibitory protein | CSB-EP327444FOA | Cusabio

Alternative Name(s): Beta-lactamase inhibitory protein; BLIP

Gene Names: N/A

Research Areas: Others

Organism: Streptomyces clavuligerus

AA Sequence: AGVMTGAKFTQIQFGMTRQQVLDIAGAENCETGGSFGDSIHCRGHAAGDYYAYATFGFTSAAADAKVDSKSQEKLLAPSAPTLTLAKFNQVTVGMTRAQVLATVGQGSCTTWSEYYPAYPSTAGVTLSLSCFDVDGYSSTGFYRGSAHLWFTDGVLQGKRQWDLV

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 37-201aa

Sequence Info: Full Length of Mature Protein

MW: 33.5 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Inhibits a wide variety of beta lactamases.

Reference: "A potent new mode of beta-lactamase inhibition revealed by the 1.7 A X-ray crystallographic structure of the TEM-1-BLIP complex." Strynadka N.C.J., Jensen S.E., Alzari P.M., James M.N.G. Nat. Struct. Biol. 3:290-297(1996)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Inhibits a wide variety of beta lactamases.

Involvement in disease:

Subcellular Location: Secreted

Protein Families:

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P35804

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: N/A

OMIM Database Link: N/A