Recombinant Streptococcus pyogenes Streptopain (speB) | CSB-EP314886SMR
- 3 - 7 Working Days
Recombinant Streptococcus pyogenes Streptopain (speB) | CSB-EP314886SMR | Cusabio
Alternative Name(s): Exotoxin type B SPE B Streptococcal cysteine proteinase Streptococcus peptidase A Short name: SPP
Gene Names: speB
Research Areas: Microbiology
Organism: Streptococcus pyogenes
AA Sequence: QPVVKSLLDSKGIHYNQGNPYNLLTPVIEKVKPGEQSFVGQHAATGCVATATAQIMKYHNYPNKGLKDYTYTLSSNNPYFNHPKNLFAAISTRQYNWNNILPTYSGRESNVQKMAISELMADVGISVDMDYGPSSGSAGSSRVQRALKENFGYNQSVHQINRSDFSKQDWEAQIDKELSQNQPVYYQGVGKVGGHAFVIDGADGRNFYHVNWGWGGVSDGFFRLDALNPSALGTGGGAGGFNGYQSAVVGIKP
Tag Info: N-terminal 6xHis-SUMO-tagged
Expression Region: 146-398aa
Sequence Info: Full Length of Mature Protein
MW: 43.6 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Important streptococcal virulence factor which cleaves human fibronectin and degrades vitronectin. Also cleaves human IL1B precursor to form biologically active IL1B. Can induce apoptosis in human monocytes and epithelial cells in vitro, and reduces phagocytic activity in monocytic cells. Thus, may play a role in bacterial colonization, invasion, and inhibition of wound healing
Reference: "A conserved Streptococcus pyogenes extracellular cysteine protease cleaves human fibronectin and degrades vitronectin."Kapur V., Topouzis S., Majesky M.W., Li L.L., Hamrick M.R., Hamill R.J., Patti J.M., Musser J.M.Microb. Pathog. 15:327-346(1993)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Important streptococcal virulence factor which cleaves human fibronectin and degrades vitronectin. Also cleaves human IL1B precursor to form biologically active IL1B. Can induce apoptosis in human monocytes and epithelial cells in vitro, and reduces phagocytic activity in monocytic cells. Thus, may play a role in bacterial colonization, invasion, and inhibition of wound healing.
Involvement in disease:
Subcellular Location: Secreted
Protein Families: Peptidase C10 family
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P0C0J0
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: N/A
STRING Database Link: N/A
OMIM Database Link: N/A