Recombinant Staphylococcus aureus Glycyl-glycine endopeptidase lytM (lytM) | CSB-EP520167FLF
- 3 - 7 Working Days
Recombinant Staphylococcus aureus Glycyl-glycine endopeptidase lytM (lytM) | CSB-EP520167FLF | Cusabio
Alternative Name(s): Autolysin LytM
Gene Names: lytM
Research Areas: others
Organism: Staphylococcus aureus (strain NCTC 8325)
AA Sequence: AETTNTQQAHTQMSTQSQDVSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANANGSGQTYVNPATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGNGKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMPENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGDQIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR
Tag Info: N-terminal 6xHis-tagged
Expression Region: 26-316aa
Sequence Info: Full Length of Mature Protein
MW: 35.9 kDa
Purity: Greater than 85% as determined by SDS-PAGE.
Relevance: Peptidoglycan hydrolase (autolysin) specifically acting on polyglycine interpeptide bridges of the cell wall peptidoglycan. Catalytic activity Hydrolysis of the -Gly-|-Gly- bond in the pentaglycine inter-peptide link joining staphylococcal cell wall peptidoglycans. EC:188.8.131.52
Reference: "Molecular cloning, sequencing, and expression of lytM, a unique autolytic gene of Staphylococcus aureus." Ramadurai L., Jayaswal R.K. J. Bacteriol. 179:3625-3631(1997)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Involvement in disease:
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: O33599
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: N/A
STRING Database Link: N/A
OMIM Database Link: N/A