Recombinant Schizosaccharomyces pombe NADH kinase pos5, mitochondrial (pos5) | CSB-YP891853SXV

Recombinant Schizosaccharomyces pombe NADH kinase pos5, mitochondrial (pos5) | CSB-YP891853SXV | Cusabio

Alternative Name(s): pos5; SPAC323.01c; NADH kinase pos5; mitochondrial; EC 2.7.1.86

Gene Names: pos5

Research Areas: Microbiology

Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

AA Sequence: NSVYSKSIRLVNTLENRIVPVYKECASPQSIGGKSNLKQLQWPKPPKNILILKKRMDERVDHCFETLVQHLQQTYPDICIITETDVAKKFSYLNLYTWTEISDLEQKVDAIITVGGDGTILHAASLFARSGMPPILSFSLGTLGFLLPFDFGSFQTAFADFYNSRSFVLMRMRLRVAMKTKLYNESIYAMNEMHIHRGLSPHMAVLKVFVNDKFLTEAVADGLIISTPTGSTAYSLSSGGPIVHPSINALLLTPICPNSLSFRPVLFPDTFKISIETSNKSRVRPQLSIDGRPLGLTDIGQRIDITSVKDNAIPCIIRSHKEDDWVSDIVSLLRWNHPFHRKGW

Source: Yeast

Tag Info: N-terminal 6xHis-tagged

Expression Region: 43-386aa

Sequence Info: Full Length of Mature Protein

MW: 40.7 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Phosphorylates both NADH and NAD+, with a preference for NADH. Anti-oxidant factor and key source of the cellular reductant NADPH

Reference: "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.Nat. Biotechnol. 24:841-847(2006)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Phosphorylates both NADH and NAD(+), with a preference for NADH. Anti-oxidant factor and key source of the cellular reductant NADPH (By similarity).

Involvement in disease:

Subcellular Location: Mitochondrion

Protein Families: NAD kinase family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q9UT98

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A