Recombinant Saccharomyces cerevisiae Cytosine deaminase (FCY1) | CSB-EP613203SVG
- 13 - 23 Working Days
Recombinant Saccharomyces cerevisiae Cytosine deaminase (FCY1) | CSB-EP613203SVG | Cusabio
Alternative Name(s): Cytosine aminohydrolase
Gene Names: FCY1
Research Areas: Others
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
AA Sequence: MVTGGMASKWDQKGMDIAYEEAALGYKEGGVPIGGCLINNKDGSVLGRGHNMRFQKGSATLHGEISTLENCGRLEGKVYKDTTLYTTLSPCDMCTGAIIMYGIPRCVVGENVNFKSKGEKYLQTRGHEVVVVDDERCKKIMKQFIDERPQDWFEDIGE
Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged
Expression Region: 1-158aa
Sequence Info: Full Length
MW: 25.0 kDa
Purity: Greater than 85% as determined by SDS-PAGE.
Relevance: Converts cytosine to uracil or 5-methylcytosine to thymine by deaminating carbon number 4.
Reference: "Isolation of thermally stable cytosine deaminase from baker's yeast." Senter P.D., Su P.C.D., Marquardt H., Hayden M.S., Linsley P.S. Submitted (AUG-1997)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Involvement in disease:
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: Q12178
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: N/A
STRING Database Link: N/A
OMIM Database Link: N/A