Recombinant Saccharomyces cerevisiae Alpha-1, 3-mannosyltransferase MNT3 (MNT3), partial | CSB-EP328134SVG1

Recombinant Saccharomyces cerevisiae Alpha-1, 3-mannosyltransferase MNT3 (MNT3), partial | CSB-EP328134SVG1 | Cusabio

Alternative Name(s): MNT3; YIL014W; Alpha-1,3-mannosyltransferase MNT3; EC 2.4.1.-

Gene Names: MNT3

Research Areas: Others

Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

AA Sequence: SRNVTSSNKLNNHASERTAVESSAFNWIEKRQHQVRSENLMNRLSAYFLPFLSRSSHKERVLLRQLGNNEIAKSDKCRYIFEVLYKIDPDWDNAQTAKFYNVDGVDNTLASLLGERLRSYDYCFLSGQLDPTAIFANSTVNPHDLQNRMFPFLKKINEESKTVMWPIITDMTTGEAVPAPEVDMESSNFNGNFWSNWNRLSKGRGFVLTIAEKDVPLFLKQLKVMEFSKNELPFQIVSTGNELSAESIAKISETAKETEQRVYLVDCSTVLDTNFANTYISFFQNKWVATLFNTFEEYILLDADVVPFVGSDYFFDSPSYRESGILLFKDRVMENEQTFQYCIEMLNEVEPSAQERRFIGSRLVFDSSLPFSSETSEEASVYYNFFKKLRLHHVDSGLVVVNKLEKLNGLLMSFMLNLDGKLQRCVYGDKEIFWLGQLYAGQDYSINPVDGSIIGPVNEEPENDDGHKSGMYYICSTQIAHSDSKNRLLWVNGGLKTCKISNSAEDDFGREPEYFKSRYGDISKLKRIYDASLNVEGLIVPDVSVHPWMQIKECSNYMYCAYATGDGHTNSELDEGRLITFTEKELRYINDISRTWNAN

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 32-630aa

Sequence Info: Partial

MW: 74.7 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans.

Reference: "Mnt2p and Mnt3p of Saccharomyces cerevisiae are members of the Mnn1p family of alpha-1,3-mannosyltransferases responsible for adding the terminal mannose residues of O-linked oligosaccharides." Romero P.A., Lussier M., Veronneau S., Sdicu A.-M., Herscovics A., Bussey H. Glycobiology 9:1045-1051(1999)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function:

Involvement in disease:

Subcellular Location:

Protein Families:

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P40549

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A