Recombinant RatTryptophan 2, 3-dioxygenaseUniRule annotation (Tdo2) | CSB-EP023351RA

Recombinant RatTryptophan 2, 3-dioxygenaseUniRule annotation (Tdo2) | CSB-EP023351RA | Cusabio

Alternative Name(s): Tryptamin 2,3-dioxygenase Tryptophan oxygenase Tryptophan pyrrolase Tryptophanase

Gene Names: Tdo2

Research Areas: Metabolism

Organism: Rattus norvegicus (Rat)

AA Sequence: MSGCPFSGNSVGYTLKNLSMEDNEEDGAQTGVNRASKGGLIYGDYLQLEKILNAQELQSEIKGNKIHDEHLFIITHQAYELWFKQILWELDSVREIFQNGHVRDERNMLKVMTRMHRVVVIFKLLVQQFSVLETMTALDFNDFREYLSPASGFQSLQFRLLENKIGVLQSLRVPYNRKHYRDNFEGDYNELLLKSEQEQTLLQLVEAWLERTPGLEPHGFNFWGKFEKNILKGLEEEFLKIQAKKDSEEKEEQMAEFRKQKEVLLCLFDEKRHDYLLSKGERRLSYRALQGALMIYFYREEPRFQVPFQLLTSLMDIDTLMTKWRYNHVCMVHRMLGSKAGTGGSSGYYYLRSTVSDRYKVFVDLFNLSSYLVPRHWIPKMNPIIHKFLYTAEYSDSSYFSSDESD

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 1-406aa

Sequence Info: Full Length

MW: 63.9 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Incorporates oxygen into the indole moiety of tryptophan. Has a broad specificity towards tryptamine and derivatives including D- and L-tryptophan, 5-hydroxytryptophan and serotonin.

Reference: "Nucleotide sequence of a fragment of the rat tryptophan oxygenase gene showing high affinity to glucocorticoid receptor in vitro."Merkulov V.M., Merkulova T.I.Biochim. Biophys. Acta 1132:100-102(1992)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Heme-dependent dioxygenase that catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring and converts L-tryptophan to N-formyl-L-kynurenine. Catalyzes the oxidative cleavage of the indole moiety.

Involvement in disease:

Subcellular Location:

Protein Families: Tryptophan 2,3-dioxygenase family

Tissue Specificity: Liver.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P21643

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A