Recombinant Rat Glucagon-like peptide 1 receptor (Glp1r), partial | CSB-YP009514RA
- 25 - 35 Working Days
Recombinant Rat Glucagon-like peptide 1 receptor (Glp1r), partial | CSB-YP009514RA | Cusabio
Alternative Name(s): Glpr
Gene Names: Glp1r
Research Areas: Cardiovascular
Organism: Rattus norvegicus (Rat)
AA Sequence: GPRPQGATVSLSETVQKWREYRHQCQRFLTEAPLLATGLFCNRTFDDYACWPDGPPGSFVNVSCPWYLPWASSVLQGHVYRFCTAEGIWLHKDNSSLPWRDLSECEESKQGERNSPEEQLLSLY
Tag Info: N-terminal 6xHis-tagged
Expression Region: 22-145aa
Sequence Info: Partial
MW: 16.3 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: G-protein coupled receptor for glucagon-like peptide 1 (GLP-1). Ligand binding triggers activation of a signaling cascade that leads to the activation of adenylyl cyclase and increased intracellular cAMP levels. Plays a role in regulating insulin secretion in response to GLP-1
Reference: "Mutations to the third cytoplasmic domain of the glucagon-like peptide 1 (GLP-1) receptor can functionally uncouple GLP-1-stimulated insulin secretion in HIT-T15 cells." Salapatek A.M., MacDonald P.E., Gaisano H.Y., Wheeler M.B. Unknown Journal 13:1305-1317(1999)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: G-protein coupled receptor for glucagon-like peptide 1 (GLP-1)
Involvement in disease:
Subcellular Location: Cell membrane, Multi-pass membrane protein
Protein Families: G-protein coupled receptor 2 family
Tissue Specificity: Pancreatic islets, stomach, lung, rat insulinoma cell line.
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P32301
HGNC Database Link: N/A
UniGene Database Link: UniGene
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: N/A