Recombinant Ralstonia pickettii Poly (3-hydroxybutyrate) depolymerase | CSB-EP319436BXV

Recombinant Ralstonia pickettii Poly (3-hydroxybutyrate) depolymerase | CSB-EP319436BXV | Cusabio

Alternative Name(s): Poly(3-hydroxybutyrate) depolymerase; PHB depolymerase; EC 3.1.1.75

Gene Names: N/A

Research Areas: Others

Organism: Ralstonia pickettii (Burkholderia pickettii)

AA Sequence: ATAGPGAWSSQQTWAADSVNGGNLTGYFYWPASQPTTPNGKRALVLVLHGCVQTASGDVIDNANGAGFNWKSVADQYGAVILAPNATGNVYSNHCWDYANASPSRTAGHVGVLLDLVNRFVTNSQYAIDPNQVYVAGLSSGGGMTMVLGCIAPDIFAGIGINAGPPPGTTTAQIGYVPSGFTATTAANKCNAWAGSNAGKFSTQIAGAVWGTSDYTVAQAYGPMDAAAMRLVYGGNFTQGSQVSISGGGTNTPYTDSNGKVRTHEISVSGMAHAWPAGTGGDNTNYVDATHINYPVFVMDYWVKNNLRAGSGTGQAGSAPTGLAVTATTSTSVSLSWNAVANASSYGVYRNGSKVGSATATAYTDSGLIAGTTYSYTVTAVDPTAGESQPSAAVSATTKSAFTCTATTASNYAHVQAGRAHDSGGIAYANGSNQSMGLDNLFYTSTLAQTAAGYYIVGNCP

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 28-488aa

Sequence Info: Full Length of Mature Protein

MW: 50.9 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: This protein degrades water-insoluble and water-soluble PHB to monomeric D--3-hydroxybutyrate.

Reference: "Proposed acquisition of an animal protein domain by bacteria." Bork P., Doolittle R.F. Proc. Natl. Acad. Sci. U.S.A. 89:8990-8994(1992)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function:

Involvement in disease:

Subcellular Location:

Protein Families:

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P12625

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A