Recombinant Pseudomonas sp. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) | CSB-EP358300PVU

Recombinant Pseudomonas sp. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) | CSB-EP358300PVU | Cusabio

Alternative Name(s): 5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase) (EPSPS)

Gene Names: aroA

Research Areas: Others

Organism: Pseudomonas sp. (strain PG2982)

AA Sequence: MSHSASPKPATARRSEALTGEIRIPGDKSISHRSFMFGGLASGETRITGLLEGEDVINTGRAMQAMGAKIRKEGDVWIINGVGNGCLLQPEAALDFGNAGTGARLTMGLVGTYDMKTSFIGDASLSKRPMGRVLNPLREMGVQVEAADGDRMPLTLIGPKTANPITYRVPMASAQVKSAVLLAGLNTPGVTTVIEPVMTRDHTEKMLQGFGADLTVETDKDGVRHIRITGQGKLVGQTIDVPGDPSSTAFPLVAALLVEGSDVTIRNVLMNPTRTGLILTLQEMGADIEVLNARLAGGEDVADLRVRASKLKGVVVPPERAPSMIDEYPVLAIAASFAEGETVMDGLDELRVKESDRLAAVARGLEANGVDCTEGEMSLTVRGRPDGKGLGGGTVATHLDHRIAMSFLVMGLAAEKPVTVDDSNMIATSFPEFMDMMPGLGAKIELSIL

Source: E.coli

Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged

Expression Region: 1-449aa

Sequence Info: Full Length

MW: 54.7 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate to the 5-hydroxyl of shikimate-3-phosphate to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.

Reference: "Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases." Barry G.F., Kishore G.M., Padgette S.R., Stallings W.C. Patent number US5633435, 27-MAY-1997

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function:

Involvement in disease:

Subcellular Location:

Protein Families:

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P0A2Y4

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A