Cusabio Pseudomonas aeruginosa Recombinants
Recombinant Pseudomonas aeruginosa Beta-lactamase OXA-10 (bla) | CSB-EP321251EZW
- 13 - 23 Working Days
Recombinant Pseudomonas aeruginosa Beta-lactamase OXA-10 (bla) | CSB-EP321251EZW | Cusabio
Alternative Name(s): Beta-lactamase PSE-2
Gene Names: bla
Research Areas: Microbiology
Organism: Pseudomonas aeruginosa
AA Sequence: SITENTSWNKEFSAEAVNGVFVLCKSSSKSCATNDLARASKEYLPASTFKIPNAIIGLETGVIKNEHQVFKWDGKPRAMKQWERDLTLRGAIQVSAVPVFQQIAREVGEVRMQKYLKKFSYGNQNISGGIDKFWLEGQLRISAVNQVEFLESLYLNKLSASKENQLIVKEALVTEAAPEYLVHSKTGFSGVGTESNPGVAWWVGWVEKETEVYFFAFNMDIDNESKLPLRKSIPTKIMESEGIIGG
Tag Info: N-terminal 6xHis-SUMO-tagged
Expression Region: 21-266aa
Sequence Info: Full Length of Mature Protein
MW: 43.5 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Hydrolyzes both carbenicillin and oxacillin.
Reference: "Insights into class D beta-lactamases are revealed by the crystal structure of the OXA10 enzyme from Pseudomonas aeruginosa."Maveyraud L., Golemi D., Kotra L.P., Tranier S., Vakulenko S., Mobashery S., Samama J.-P.Structure 8:1289-1298(2000)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Hydrolyzes both carbenicillin and oxacillin.
Involvement in disease:
Protein Families: Class-D beta-lactamase family
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P14489
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: KEGG
STRING Database Link: N/A
OMIM Database Link: N/A