Recombinant Pseudomonas aeruginosa Beta-lactamase OXA-10 (bla) | CSB-EP321251EZW

Recombinant Pseudomonas aeruginosa Beta-lactamase OXA-10 (bla) | CSB-EP321251EZW | Cusabio

Alternative Name(s): Beta-lactamase PSE-2

Gene Names: bla

Research Areas: Microbiology

Organism: Pseudomonas aeruginosa

AA Sequence: SITENTSWNKEFSAEAVNGVFVLCKSSSKSCATNDLARASKEYLPASTFKIPNAIIGLETGVIKNEHQVFKWDGKPRAMKQWERDLTLRGAIQVSAVPVFQQIAREVGEVRMQKYLKKFSYGNQNISGGIDKFWLEGQLRISAVNQVEFLESLYLNKLSASKENQLIVKEALVTEAAPEYLVHSKTGFSGVGTESNPGVAWWVGWVEKETEVYFFAFNMDIDNESKLPLRKSIPTKIMESEGIIGG

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 21-266aa

Sequence Info: Full Length of Mature Protein

MW: 43.5 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Hydrolyzes both carbenicillin and oxacillin.

Reference: "Insights into class D beta-lactamases are revealed by the crystal structure of the OXA10 enzyme from Pseudomonas aeruginosa."Maveyraud L., Golemi D., Kotra L.P., Tranier S., Vakulenko S., Mobashery S., Samama J.-P.Structure 8:1289-1298(2000)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Hydrolyzes both carbenicillin and oxacillin.

Involvement in disease:

Subcellular Location:

Protein Families: Class-D beta-lactamase family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P14489

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: N/A

OMIM Database Link: N/A