Recombinant Pongo abelii C-1-tetrahydrofolate synthase, Cytoplasmic domain (MTHFD1), partial | CSB-EP732682PYX

Recombinant Pongo abelii C-1-tetrahydrofolate synthase, Cytoplasmic domain (MTHFD1), partial | CSB-EP732682PYX | Cusabio

Alternative Name(s): Short name:C1-THF synthase

Gene Names: MTHFD1

Research Areas: Cell Biology

Organism: Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)

AA Sequence: MAPAEILNGREISAQIRARLKNQVTQLKEQVPGFTPGLAILLVGNRDDSNLYINVKLKAAEEIGIKATHIKLPRTTTESEVIKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVDGLTSISAGKLARGDLNDCFIPCTPKGCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTANLDEEVNKGDILVVATGRPEMVKGEWIKPGAIVIDCGINYVPDDKKPNGRKVVGDVAYDEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKPGKWMI

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 1-305aa

Sequence Info: Partial

MW: 49 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: 5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH. 5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate. ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.

Reference: The German cDNA consortium Submitted (NOV-2004)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function:

Involvement in disease:

Subcellular Location: Cytoplasm

Protein Families: Tetrahydrofolate dehydrogenase/cyclohydrolase family; Formate--tetrahydrofolate ligase family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q5R8P0

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: N/A

OMIM Database Link: N/A