Cusabio Pongo abelii Recombinants
Recombinant Pongo abelii C-1-tetrahydrofolate synthase, Cytoplasmic domain (MTHFD1), partial | CSB-EP732682PYX
- SKU:
- CSB-EP732682PYX
- Availability:
- 13 - 23 Working Days
Description
Recombinant Pongo abelii C-1-tetrahydrofolate synthase, Cytoplasmic domain (MTHFD1), partial | CSB-EP732682PYX | Cusabio
Alternative Name(s): Short name:C1-THF synthase
Gene Names: MTHFD1
Research Areas: Cell Biology
Organism: Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
AA Sequence: MAPAEILNGREISAQIRARLKNQVTQLKEQVPGFTPGLAILLVGNRDDSNLYINVKLKAAEEIGIKATHIKLPRTTTESEVIKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVDGLTSISAGKLARGDLNDCFIPCTPKGCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTANLDEEVNKGDILVVATGRPEMVKGEWIKPGAIVIDCGINYVPDDKKPNGRKVVGDVAYDEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKPGKWMI
Source: E.coli
Tag Info: N-terminal 6xHis-SUMO-tagged
Expression Region: 1-305aa
Sequence Info: Partial
MW: 49 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: 5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH. 5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate. ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.
Reference: The German cDNA consortium Submitted (NOV-2004)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function:
Involvement in disease:
Subcellular Location: Cytoplasm
Protein Families: Tetrahydrofolate dehydrogenase/cyclohydrolase family; Formate--tetrahydrofolate ligase family
Tissue Specificity:
Paythway:
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: Q5R8P0
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: KEGG
STRING Database Link: N/A
OMIM Database Link: N/A