Recombinant Poecilobdella viridis Hirudin (Active) | CSB-AP000241PNX
- 5 to 10 Working Days
Recombinant Poecilobdella viridis Hirudin (Active) | CSB-AP000241PNX | Cusabio
Protein Description: Full Length
Alternative Name (s) :
Gene Names: N/A
Research Areas: Neuroscience
Species: Poecilobdella viridis (Indian freshwater leech)
Tag Info: Tag-Free
Expression Region: 1-63aa
Sequence Info: VVYTDCTESG QNLCLCEGSN VCGQGNKCIL GSDGEKNQCV TGEGTPGPQS HNDGDFEEPE EYL
Biological Activity: The biological activity is determined by chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific activity is no less than 14,000 ATU/mg protein.
MW: 6.7 kDa
Purity: >96% as determined by SDS-PAGE and HPLC.
Endotoxin: Less than 1.0 EU/µg as determined by LAL method.
Relevance: Hirudin is a potent thrombin-specific protease inhibitor. It forms a stable non-covalent complex with alpha-thrombin, thereby abolishing its ability to cleave fibrinogen.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Function: Hirudin is a potent thrombin-specific protease inhibitor. It forms a stable non-covalent complex with alpha-thrombin, thereby abolishing its ability to cleave fibrinogen.
Involvement in disease:
Subcellular Location: Secreted
Protein Families: Protease inhibitor I14 (hirudin) family
Form: Lyophilized powder
Buffer: Lyophilized from a 0.2 μm filtered 20 mM PBS, pH 7.0, containing 2 % mannitol
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P84590
Uniprot Entry Name: HIRUD_POEVI
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: N/A
STRING Database Link: N/A
OMIM Database Link: N/A