Recombinant Oryza sativa subsp. japonica Ubiquitin-conjugating enzyme E2 5A (UBC5A) | CSB-EP848293OFG

Recombinant Oryza sativa subsp. japonica Ubiquitin-conjugating enzyme E2 5A (UBC5A) | CSB-EP848293OFG | Cusabio

Alternative Name(s): E2 ubiquitin-conjugating enzyme 5A; Ubiquitin carrier protein 5a; OsUBC5a; Ubiquitin-protein ligase 5A

Gene Names: UBC5A

Research Areas: Cell Biology

Organism: Oryza sativa subsp. japonica (Rice)

AA Sequence: MASKRIQKELKDLQKDPPTSCSAGPVGEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKDQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRHKYENTARTWTQRYAM

Source: E.coli

Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged

Expression Region: 1-147aa

Sequence Info: Full Length

MW: 23.6 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: E2 conjugating enzyme that associates with the E3 ubiquitin-protein ligase EL5 to mediates ubiquitination of target proteins.

Reference:

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: E2 conjugating enzyme that associates with the E3 ubiquitin-protein ligase EL5 to mediates ubiquitination of target proteins.

Involvement in disease:

Subcellular Location:

Protein Families: Ubiquitin-conjugating enzyme family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q8S920

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A