Recombinant Mouse Tryptase beta-2 (Tpsb2) | CSB-EP024128MO
- 3 - 7 Working Days
Recombinant Mouse Tryptase beta-2 (Tpsb2) | CSB-EP024128MO | Cusabio
Alternative Name(s): Mast cell protease 6 ;mMCP-6
Gene Names: Tpsb2
Research Areas: Others
Organism: Mus musculus (Mouse)
AA Sequence: IVGGHEASESKWPWQVSLRFKLNYWIHFCGGSLIHPQWVLTAAHCVGPHIKSPQLFRVQLREQYLYYGDQLLSLNRIVVHPHYYTAEGGADVALLELEVPVNVSTHIHPISLPPASETFPPGTSCWVTGWGDIDNDEPLPPPYPLKQVKVPIVENSLCDRKYHTGLYTGDDFPIVHDGMLCAGNTRRDSCQGDSGGPLVCKVKGTWLQAGVVSWGEGCAQPNKPGIYTRVTYYLDWIHRYVPEHS
Tag Info: N-terminal GST-tagged
Expression Region: 32-276aa
Sequence Info: Full Length of Mature Protein
MW: 54.4 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Has an immunoprotective role during bacterial infection. Required to efficiently combat K.pneumoniae infection.
Reference: The expression pattern of three mast cell specific proteases during mouse development.Abraham D., Oster H., Huber M., Leitges M.Mol. Immunol. 44:732-740(2007)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Plays a role in innate immunity.
Involvement in disease:
Subcellular Location: Secreted
Protein Families: Peptidase S1 family, Tryptase subfamily
Tissue Specificity: During embryogenesis, detected primarily in skin.
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P21845
HGNC Database Link: N/A
UniGene Database Link: UniGene
KEGG Database Link: N/A
STRING Database Link: STRING
OMIM Database Link: N/A