Recombinant Mouse Sushi domain-containing protein 4 (Susd4), partial | CSB-EP805887MOa0

Recombinant Mouse Sushi domain-containing protein 4 (Susd4), partial | CSB-EP805887MOa0 | Cusabio

Alternative Name(s): Susd4; Sushi domain-containing protein 4

Gene Names: Susd4

Research Areas: Cell Biology

Organism: Mus musculus (Mouse)

AA Sequence: FGPAQLTGGFDDLNVCADPGVPENGFRTPSGGVFFESSVTRFHCQDGFRLKGSTKRLCMKHFNGTLGWVPSDKPVCIQEDCRIPQIEDAEIRNKTYRHGEKLVIDCHEGFKIRYPDLYNLVSLCRDDGTWDNLPICQGCLRPLASSNGYVNISEFQTSFPVGTVIAYRCFPGFKLEGSENLECLHNLIWSSSPPRCLALEVCPLPPMVSHGDFICHPRPCERYNHGTVVEFYCDPGYSLTSDYKYITCQYGEWFPSYQVYCIKSEQTWPSTHETLLTT

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 42-319aa

Sequence Info: Partial

MW: 35.5 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Acts as complement inhibitor by disrupting the formation of the classical C3 convertase. Isoform 3 inhibits the classical complement pathway, while membrane-bound isoform 1 inhibits deposition of C3b via both the classical and alternative complement pathways.

Reference: "Quantitative Trait Locus Mapping of Macrophage Cholesterol Metabolism and CRISPR/Cas9 Editing Implicate an ACAT1 Truncation as a Causal Modifier Variant." Hai Q., Ritchey B., Robinet P., Alzayed A.M., Brubaker G., Zhang J., Smith J.D. Arterioscler. Thromb. Vasc. Biol. 38:83-91(2018)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Acts as complement inhibitor by disrupting the formation of the classical C3 convertase. Isoform 3 inhibits the classical complement pathway, while membrane-bound isoform 1 inhibits deposition of C3b via both the classical and alternative complement pathways.

Involvement in disease:

Subcellular Location: Membrane, Single-pass type I membrane protein

Protein Families:

Tissue Specificity: High expression in brain and eye, with weaker expression in spinal cord and testis. Detected in white matter of brain and in the outer segments of photoreceptors.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q8BH32

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A