Cusabio Mouse Recombinants
Recombinant Mouse Protein Dihydroorotate dehydrogenase (quinone), mitochondrial (Dhodh) | CSB-CF006852MO
- 3 - 7 Working Days
Recombinant Mouse Protein Dihydroorotate dehydrogenase (quinone), mitochondrial (Dhodh) | CSB-CF006852MO | Cusabio
Alternative Name(s): Dihydroorotate oxidase
Gene Names: Dhodh
Research Areas: Metabolism
Organism: Mus musculus (Mouse)
AA Sequence: LDAAIILGGGGLLFTSYLTATGDDHFYAEYLMPALQRLLDPESAHRLAVRVISLGLLPRATFQDSNMLEVRVLGHKFRNPVGIAAGFDKHGEAVDGLYKLGFGFVEVGSVTPQPQEGNPRPRVFRLPEDQAVINRYGFNSHGLSAVEHRLRARQQKQTQLTTDGLPLGINLGKNKTSVDAAADYVEGVRILGPLADYLVVNVSSPNTAGLRSLQGKTELRRLLSKVLQERDALKGPQKPAVLVKIAPDLTAQDKEDIASVARELGIDGLIITNTTVSRPVGLQGALRSETGGLSGKPLRDLSTQTIREMYALTQGTIPIIGVGGVSSGQDALEKIQAGASLVQLYTALTFLGPPVVARVKRELEALLKERGFNTVTDAIGVDHRR
Source: in vitro E.coli expression system
Tag Info: C-terminal 6xHis-tagged
Expression Region: 11-395aa
Sequence Info: Full Length of Mature Protein
MW: 44.2 kDa
Purity: Greater than 85% as determined by SDS-PAGE.
Relevance: Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
Reference: "In vitro and in Vivo inhibition of immunoglobulin secretion by the immunosuppressive compound HR325 is reversed by exogenous uridine." Thomson T.A., Spinella-Jaegle S., Francesconi E., Meakin C., Millet S., Flao K.L., Hidden H., Ruuth E. Scand. J. Immunol. 56:35-42(2002)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
Involvement in disease:
Subcellular Location: Mitochondrion inner membrane, Single-pass membrane protein
Protein Families: Dihydroorotate dehydrogenase family, Type 2 subfamily
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: O35435
HGNC Database Link: N/A
UniGene Database Link: UniGene
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: N/A